The present study is aimed at determining the difference between respondents in terms of emotional reactivity (activation of positive emotions, intensity of positive emotions, duration of negative emotions, intensity of negative emotions, general reactivity of positive emotions, general reactivity of negative emotions) and tolerance to ambiguity (novelty of the problem, complexity of the problem, unsolvability of the problem) depending on gender. The sample consisted of 216 respondents (men = 21, women = 195) who were selected using a stratified random method. For this study, the Perth Emotional Reactivity Scale and the S. Badner’s uncertainty tolerance scale were used. The data was distributed normally, and non-parametric analysis was used. The results revealed statistically significant differences in the level of emotional reactivity (activation of positive emotions, intensity of positive emotions, intensity of negative emotions, general reactivity of positive emotions, general reactivity of negative emotions) and tolerance to uncertainty (novelty of the problem, complexity of the problem, unsolvability of the problem) depending on gender (men and women). And according to the factor of emotional reactivity, there were no statistically significant differences by gender.