The article highlights the problem of gender peculiarities of translational-informational functional component of Ukrainian civil servants’ professional communication culture. On analyzing the results of empirical research, it can be concluded that civil servants’ professional communication culture has significant gender differences in some indicators. Among male civil servants, the most common is the average level of psychological literacy in professional communication and the identical level of psychological competence in professional communication. The majority of female civil servants have a high level of psychological literacy as well as psychological competence in professional communication. Only female civil servants’ orientation in professional communication correlates with the acmeological invariants of the personality’s professionalism in business communication.